FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094
Total Bases158.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC28726.23400365630713No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC19517.824497257769654No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG524.753199268738574No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT474.296160877513711No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG373.3820840950639854No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA262.376599634369287No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT181.6453382084095063No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC141.2797074954296161No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT131.1882998171846435No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC80.7312614259597806No Hit
CGAAGTAGACCAGCGGCATCACCATCCACCTTTTGCCATGTAGAACCATG70.6398537477148081No Hit
GTTGGCGGATACCCTTGGGGCGTAAGGGACCGAAATATAAGATGTGGCAA70.6398537477148081No Hit
GTGTCGCTACCTCAGTTTGCACACTGACCACATCACCATCCACCTTTTGC60.5484460694698354No Hit
GTTGGCGGATACCCTCGGGGCGTAAGGGACCGAAATATAAGATGTGGCAA60.5484460694698354No Hit
TACCTCAGTTTGCCCCCATGGGCTCGTGTCGCCGGTGTAGCCCCCGCGGT60.5484460694698354No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTACAGATGTGG60.5484460694698354No Hit
GTTGGCGGATACCCTTCCAGATGTGGCAAGATGGCATTGCCATGTAGAAC60.5484460694698354No Hit
GTTGGCGGATACCCTGATGTGGCAAGATGGCATTGCCATGTAGAACCATG60.5484460694698354No Hit
GTTGGCGGATACCAAGATGTGGCAAGATGGCATTGCCATGTAGAACCATG60.5484460694698354No Hit
TACCTCAGTTTGCCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGT50.4570383912248629No Hit
GTTGGCGGATACCCTAAGATGTGGCAAGATGGCATTGCCATGTAGAACCA50.4570383912248629No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGACCACATC50.4570383912248629Clontech Universal Primer Mix Long (96% over 27bp)
GTTGGCGGATACCCAGATGTGGCAAGATGGCATTGCCATGTAGAACCATG40.3656307129798903No Hit
GTTGGCGGATACCCTTGTTGAGCCAGCATTAGCAGCTGGTACAGATGTGG40.3656307129798903No Hit
TACTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG40.3656307129798903No Hit
GTTGGCGGATACCCCTAAGATGTGGCAAGATGGCATTGCCATGTAGAACC40.3656307129798903No Hit
GTTGGCGGATACCCTTCCTGCCTTCAAGATGTGGCAAGATGGCATTGCCA40.3656307129798903No Hit
GTTGGCGGATACCCAAGATGTGGCAAGATGGCATTGCCATGTAGAACCAT30.2742230347349177No Hit
TACCTCAGTTTGCCCCCACCATAAGATGTCCTCTTGCCATGTAGAACCAT30.2742230347349177No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACCCCATGTACTCTGC30.2742230347349177Clontech SMART CDS Primer II A (95% over 21bp)
TACCTCAGTTTGCCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGT30.2742230347349177No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTGCTGTGTTGGCGGAT30.2742230347349177No Hit
TACCTCAGTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG30.2742230347349177No Hit
GTTGGCGGATACCCCCCCAAGATGTGGCAAGATGGCATTGCCATGTAGAA20.18281535648994515No Hit
CGAAGTAGACCACATCACCATCCACCTTTGCCATGTAGAACCATGTCGTC20.18281535648994515No Hit
GTTGGCGGATACCCTGTGTTGGCAGATGTGGCAAGATGGCATTGCCATGT20.18281535648994515No Hit
GTTGGCGGATACCCCAAGATGTGGCAAGATGGCATTGCCATGTAGAACCA20.18281535648994515No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGAGAAAAGG20.18281535648994515Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTCCAGATGTGGCAAGATGGCATTGCCATGTAGAACC20.18281535648994515No Hit
GTTGGCGGATACCCTTCCGATGTGGCAAGATGGCATTGCCATGTAGAACC20.18281535648994515No Hit
GTTGGCGGATACCCTCCAAGATGTGGCAAGATGGCATTGCCATGTAGAAC20.18281535648994515No Hit
GTTGGCGGATACCCTAGATGTGGCAAGATGGCATTGCCATGTAGAACCAT20.18281535648994515No Hit
GTTGGCGGATACCCTTCGTGATAGATGTGGCAAGATGGCATTGCCATGTA20.18281535648994515No Hit
TACCTCAGTTTGCCCCACCATAAGATGTCCTCTTGCCATGTAGAACCATG20.18281535648994515No Hit
GTTGGCGGATACCCTCAAGATGTGGCAAGATGGCATTGCCATGTAGAACC20.18281535648994515No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGGTGGCGGAT20.18281535648994515No Hit
GTTGGCGGATACCCTTGTCTGTGTGTTGGCGGATACCCTTGTCTGTGTTG20.18281535648994515No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTGGTGTGTTGGCTGAT20.18281535648994515No Hit
TAACTCAGATACCCAACAAAATATGTCCTCTTGCCATGTAGAACCATGTC20.18281535648994515No Hit
GTTGGCGGATACCTAAGATGTGGCAAGATGGCATTGCCATGTAGAACCAT20.18281535648994515No Hit
GTTGGCGGATACCCTTACTGCCTTCAAGATGTGGCAAGATGGCATTGCCA20.18281535648994515No Hit
TAACTCAGATTCCCAACAAAAGATGTCCTCTTGCCATGTAGAACCATGTC20.18281535648994515No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGGCCTAAGA20.18281535648994515Clontech Universal Primer Mix Long (96% over 29bp)
GTTGGCGGATACCCTTGGCAGATGTGGCAAGATGGCATTGCCATGTAGAA20.18281535648994515No Hit
CGAAGTAGACCACATCACCATCCACTTTTGCCATGTAGAACCATGTCGTC20.18281535648994515No Hit
TACCTAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG20.18281535648994515No Hit
GTTGGCGGATACCCATCCATACACTGACCACATCACCATCCACCTTTTGC20.18281535648994515No Hit
TACCTCATTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG20.18281535648994515No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCATTCTGTGTTGGCGGAT20.18281535648994515No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC20.18281535648994515No Hit
ACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG20.18281535648994515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)