FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12006
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC730260.81959020489756No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG169514.117941029485257No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC3943.2816924870897886No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT1311.09112110611361No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA1040.8662335498917209No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT670.5580543061802432No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC520.4331167749458604No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT520.4331167749458604No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT470.3914709312010661No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT440.36648342495418956No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT410.341495918707313No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC400.33316674995835416No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.32483758120939527No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA360.29985007496251875No Hit
GAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT340.283191737464601No Hit
CGAAGTAGACCACGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT320.26653339996668335No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT310.25820423121772446No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT300.24987506246876562No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT290.24154589371980675No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT290.24154589371980675No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA280.23321672497084792No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCCCCCTGGACCTTAGCTCAGCAC270.22488755622188905No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA240.19990004997501248No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA230.19157088122605362No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC230.19157088122605362No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGAAC220.18324171247709478No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA210.17491254372813594No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA210.17491254372813594No Hit
CGAAGTAGACCAGCGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT210.17491254372813594No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA200.16658337497917708No Hit
CGAAGTAGACCAGCGGCACCTCGGCCCCACCCTGGACCTTAGCTCAGCAC200.16658337497917708No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC180.14992503748125938No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT160.13326669998334167No Hit
CGAAGTAGACCAGGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT160.13326669998334167No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCCGCAC150.12493753123438281No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCGGCAC150.12493753123438281No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA140.11660836248542396No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGGCCTTAGCTCAGCAC140.11660836248542396No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCCTAGCTCAGCAC140.11660836248542396No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCCGGACCTTAGCTCAGCAC140.11660836248542396No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCACCCTGGACCTTAGCTCAGCACT130.1082791937364651No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCCCAGCAC130.1082791937364651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)