FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54251
Total Bases7.8 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1960936.14495585334833No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1370425.260363864260565No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG784314.45687637094247No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC7881.452507787874878No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5531.0193360491050856No Hit
GTGTCGCTACCTCAGTTTGCCCCCGTGTCCCTTACACACACGCAAAATAC3040.5603583344085824No Hit
GTTGGCGGATACCCTTCCCCAGATTCATAGATGTGGCAAGATGGCATTGC2850.5253359385080459No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG2360.4350150227645573No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2270.41842546681167164No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1870.34469410702106873No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1810.3336344030524783No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1780.3281045510681831No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1560.28755230318335145No Hit
GTTGGCGGATACCCTTCCTTGTAGTAAGACACCAACTGCTTAAAACATAA1530.2820224511990562No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1490.2746493152199959No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1350.2488433392932849No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT1320.2433134873089897No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG1270.23409706733516436No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1260.23225378334039926No Hit
GTTGGCGGATACCCTTCCATGGCCACGTCCTAAGATGTGGCAAGATGGCA1220.22488064736133898No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT1070.19723138743986285No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1030.18985825146080257No Hit
GTTGGCGGATACCCTTCCATGGCCACCGTCCTAAGATGTGGCAAGATGGC960.17695526349744706No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA870.1603657075445614No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA830.1529925715655011No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA830.1529925715655011No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG750.13824629960738052No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG720.1327164476230853No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG720.1327164476230853No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG720.1327164476230853No Hit
GTTGGCGGATACCCTTCCCACAATCGTGTTCAAGATGTGGCAAGATGGCA710.13087316362832022No Hit
GTTGGCGGATACCCTTCCATGTAGTAAGACACCAACTGCTTAAAACATAA690.12718659563879006No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA690.12718659563879006No Hit
GTTGGCGGATACCCTTCCGGAAGATGTGGCAAGATGGCATTGCCATGTAG680.125343311644025No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA670.12350002764925992No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA600.11059703968590441No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT560.10322390370684412No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)