FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27413
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC884232.254769634844784No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC574020.938970561412468No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG25149.170831357385183No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5331.9443329807025864No Hit
GTTGGCGGATACCCTTCCATTAGCCATCAGAAAGATGTCATCATTGCATG5301.9333892678656113No Hit
GTTGGCGGATACCCTTCCCCAATCCCCCCATTTTTTGGGGCAGAAATTTT3971.4482179987597126No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3451.2585269762521432No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1710.623791631707584No Hit
GTTGGCGGATACCCTTCGTCAGCAAGACAGAAATATTCCAAGAGGAAGTG1190.4341006092000146No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1110.40491737496808083Clontech Universal Primer Mix Long (96% over 26bp)
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT1090.3976215664100974No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT1030.3757341407361471No Hit
GTTGGCGGATACCCTTCTCGCTGTGATGAGTGATTGTTAAACATTCGTAG980.3574946193411885No Hit
GTTGGCGGATACCCTTCCCCGTTGTTAACCCTTCGTACACTTCTTCGTTT710.2590012038084121No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT610.2225221610184949No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.21522635246051142No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA580.2115784481815197No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG580.2115784481815197No Hit
GTTGGCGGATACCCTTCCCGCTGTGATGAGTGATTGTTAAACATTCGTAG580.2115784481815197No Hit
GTTGGCGGATACCCTTAAGAAACACAAAAAAAGGTGCCATCTACCTCTTA560.2042826396235363No Hit
GTTGGCGGATACCCTTGCAAACAAGCTCTCTCATCTAAAGTAGTGACCTA540.19698683106555281No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA520.18969102250756942No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT500.18239521394958597No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA490.17874730967059424No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA470.17145150111261082No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA460.1678035968336191No Hit
TACCTCAGTTTGCCCCCATGGTCATTTTAGACAGATGTGGCAAGATGGCA450.16415569255462736No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC450.16415569255462736Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC410.1495640754386605No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT410.1495640754386605No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT410.1495640754386605No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC410.1495640754386605No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT400.14591617115966876No Hit
TACCTCAGTTTGCCCCCATTTTTTGGGGCAGAAATTTTTATGTCTTTATG400.14591617115966876No Hit
GTTGGCGGATACCCTTCCTACCGATCTGACTGGTTGAGATGTGGCAAGAT390.14226826688067706No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT390.14226826688067706No Hit
TACCTCAGTTTGCCCCCATGTCCACTTCAGTCATGACATTCTTCCGGGCA380.13862036260168534No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG370.1349724583226936No Hit
GTTGGCGGATACCCTTGACAGATGTGGCAAGATGGCATTGCCATGTAGAA370.1349724583226936No Hit
GTTGGCGGATACCCTAAGATGTGGCAAGATGGCATTGCCATGTAGAACCA370.1349724583226936No Hit
GTTGGCGGATACCCTTCGTCTTGGGCGAAGATGTGGCAAGATGGCATTGC360.13132455404370189No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT340.12402874548571846No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCATT340.12402874548571846No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT340.12402874548571846No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.11673293692773502No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA310.11308503264874328No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC300.10943712836975159No Hit
GTTGGCGGATACCCTTGGAAAGATGTGGCAAGATGGCATTGCCATGTAGA300.10943712836975159No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT300.10943712836975159No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT300.10943712836975159No Hit
GTTGGCGGATACCCTTCGCCCAAGGACGTAGATGTGGCAAGATGGCATTG300.10943712836975159No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT280.10214131981176815No Hit
CGAAGTAGACCACGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT280.10214131981176815No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)