FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39210
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1442036.776332568222394No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1118528.525886253506762No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT36959.423616424381535No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA17984.585564906911502No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC15683.998979852078551No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG10322.631981637337414No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG7101.810762560571283No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC1900.4845702626880897No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT1600.408059168579444No Hit
TGCCATCTTGCCACATCTGTACTAGCACAGGCTGAAGCTTTTGTATTTTA1540.39275694975771486No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC1160.2958428972200969No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC750.1912777352716144No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC410.10456516194848253No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT400.102014792144861No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)