FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17963
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA810345.10939152702778No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA443424.68407281634471No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT314917.530479318599344No Hit
TGCCATCTTGCCACATCTGCAGTGGTATCAACGCAGAGTACATGGGAAGC1420.7905138339920948Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1010.5622668819239548No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC720.4008239158269777No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT610.33958692868674495No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT440.2449479485609308No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT410.22824695206814008No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG330.1837109614206981No Hit
TGCCATCTTGCCACATCTGCAGTGGTATCAACGCAGAGTACATGGGTATC300.16700996492790737Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT290.16144296609697711No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT290.16144296609697711No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG280.15587596726604688No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.1447419696041864No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT240.1336079719423259No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA220.1224739742804654No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC220.1224739742804654No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT210.11690697544953516No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.10577297778767467No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT190.10577297778767467No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA190.10577297778767467No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTCGGTTTCCCAGTCCACTA190.10577297778767467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)