FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17165
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC714741.63705214098456No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG585634.11593358578503No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA13457.835712205068453No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA2611.5205359743664433No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1020.5942324497524031No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA870.5068453247888145No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA870.5068453247888145No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA730.42528400815613165No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.34372269152344886No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC460.267987183221672No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA440.2563355665598602No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA360.20972909991261285No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA310.1806000582580833No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC290.16894844159627148No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.16894844159627148No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC280.16312263326536555No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC250.14564520827264782No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC230.133993591610836No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT230.133993591610836No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATAC220.1281677832799301No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT220.1281677832799301No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT220.1281677832799301No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC210.12234197494902417No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC200.11651616661811826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)