FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14648
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA505434.50300382304752No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT417728.51583833970508No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA233115.913435281267066No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT9186.267067176406335No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC3342.280174767886401No Hit
TGCCATCTTGCCACATCTTGTATCAACGCAGAGTACATGGGAAGCAGTGG750.51201529219006No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT460.3140360458765702No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT420.2867285636264337No Hit
TGCCATCTTGCCACATCTGGTATCAACGCAGAGTACATGGGAAGCAGTGG420.2867285636264337No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.21163298743855818No Hit
TGCCATCTTGCCACATCTTATCAACGCAGAGTACATGGGAAGCAGTGGTA290.1979792463134899No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT250.17067176406335335No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT230.1570180229382851No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT230.1570180229382851No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT230.1570180229382851No Hit
TGCCATCTTGCCACATCTTGTATCAACGCAGAGTACATGGGAAGCAAGGG210.14336428181321684No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT200.1365374112506827No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA200.1365374112506827No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.12971054068814855No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.12971054068814855No Hit
TGCCATCTTGCCACATCTGGTATCAACGCAGAGTACATGGGTATCCGCCA170.11605679956308028No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT170.11605679956308028No Hit
TTCTGCAGTTTGGCTGAATAGTTGCTATATTGAGAGAATCTGGATGATAT160.10922992900054614No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT160.10922992900054614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)