FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17630
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA612134.71922858763472No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT451425.60408394781622No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA403622.892796369824165No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT6673.7833238797504256No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC3622.0533182076006806No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1050.5955757231990925No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT630.35734543391945545No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT530.30062393647192287No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT530.30062393647192287No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT290.1644923425978446No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT290.1644923425978446No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.15882019285309132No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.1474758933635848No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT240.13613159387407828No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT220.12478729438457174No Hit
AAGGTGGATGGTGTGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT220.12478729438457174No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC210.1191151446398185No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT200.11344299489506524No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.10777084515031198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)