FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18307
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA550430.065002458076144No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT456324.92489211776916No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA391121.36341290216857No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT14938.155350412410552No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC5923.233735729502376No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG810.44245370623258856No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT800.4369913147976184No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG540.29496913748839243No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT490.2676571803135413No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT490.2676571803135413No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT450.24580761457366035No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT400.2184956573988092No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.1966460916589283No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT300.1638717430491069No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC290.15840935161413666No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT260.14202217730922598No Hit
TGCCATCTTGCCACATCTTACTTCAGTGAAATTAAGTACCAAATTCCAGT250.13655978587425574No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT230.1256350030043153No Hit
TGCCATCTTGCCACATCTTTTCCCTGTCAGGATCAGCTTGGAACCAAAAG220.12017261156934507No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.10378543726443437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)