FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17743
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA601833.91760130755791No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT564931.837907907343745No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA356720.103702868736967No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC3331.8767964831201036No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2461.386462266809446No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT920.5185143436848335No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT620.34943357943977904No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT570.32125345206560335No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT490.2761652482669222No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT410.231077044468241No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.17471678971988952No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG250.14090063687087867No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT250.14090063687087867No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT240.1352646113960435No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT240.1352646113960435No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT230.12962858592120838No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC230.12962858592120838No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT220.12399256044637322No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT210.11835653497153807No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT210.11835653497153807No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCTGGGCTGA210.11835653497153807No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT180.10144845854703263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)