FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31698
Total Bases4.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC880127.76515868509054No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA841926.560035333459524No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT670121.140135024291755No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA350211.048015647674932No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2510.7918480661240457No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1580.49845416114581365No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT1240.39119187330430943No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT770.24291753422928894No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT650.20506025616758156No Hit
TGCCATCTTGCCACATCTTGTTTCACATTTCAGCAATAAGCCCAAAATGA650.20506025616758156No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT550.17351252444949208No Hit
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT490.1545838854186384No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT440.13881001955959366No Hit
TGCCATCTTGCCACATCTTTACATTTATTACATATAAATCTAACAATCCC400.1261909268723579No Hit
TGCCATCTTGCCACATCTACCAAGTAGATTCCATAAATGTTTCCTATTTC390.12303615370054892No Hit
TGCCATCTTCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT380.11988138052873999No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.1135718341851221No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT360.1135718341851221No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT340.1072622878415042No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG320.1009527414978863No Hit
TGCCATCTTGCCACATCTCCATGTAAGCCGAGCTACAGTACCACTGCAAA320.1009527414978863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)