FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16834
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA892553.01770226921706No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA326219.37745039800404No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT248514.761791612213376No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2791.6573601045503146No Hit
TGCCCTCTCTGCCTTTTCTCTCCCAGGGTATTTGGTTTCCCAGTCCACTA820.4871094214090531No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT790.469288345016039No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT790.469288345016039No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC550.3267197338719259No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT390.23167399310918382No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC360.21385291671616966No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.21385291671616966No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT270.16038968753712723No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG270.16038968753712723No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT240.1425686111441131No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG230.13662825234644174No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT220.13068789354877033No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA210.12474753475109897No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTCGGTTTCCCAGTCCACTA180.10692645835808483No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA170.10098609956041345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)