FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14113
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA699349.550060228158436No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT428930.39042017997591No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA7975.647275561539007No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT5353.7908311485864097No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC870.6164529157514348No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT430.30468362502657126No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTGTTTGGTTTCCCAGTCCACTA260.1842273081556012No Hit
TTCTGCAGTTTGGCTAATAGTTGATATATTGAGAGAATCTGGATGATATT230.16297031106072415No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT220.1558846453624318No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA210.14879897966413944No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT200.14171331396584708No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT200.14171331396584708No Hit
TTCTGCAGTTTGGCTGAATAGTTGCTATATTGAGAGAATCTGGATGATAT200.14171331396584708No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA200.14171331396584708No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.13462764826755472No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT190.13462764826755472No Hit
TTCTGCAGTTTGGCTGATAGTTGATATATTGAGAGAATCTGGATGATATT180.12754198256926239No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATCTGGTTTCCCAGTCCACTA170.12045631687097004No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA170.12045631687097004No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT160.11337065117267768No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT150.10628498547438532No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT150.10628498547438532No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT150.10628498547438532No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT150.10628498547438532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)