FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44456
Total Bases6.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1566735.24158718733129No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1079124.27343890588447No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT760717.111301061723953No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC32327.2701097714594205No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA22935.157908943674645No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC3280.7378081698758323No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1070.2406874212704697No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACCAGTC930.20919560914162316No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT640.14396256973186972No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT590.1327154939715674No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT560.12596724851538602No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACAGTCAA540.12146841821126507No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACCAGTCAA480.10797192729890229No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACCAGTCAA470.10572251214684182No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC460.10347309699478137No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG460.10347309699478137No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)