FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46316
Total Bases6.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC1694036.57483375075568No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1275927.547715692201397No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT666614.392434579842819No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA469910.145522065808791No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT2130.4598842732533034No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT1470.3173849209776319No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1430.3087485965972882No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1070.23102167717419467No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG850.18352189308230418No Hit
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT810.17488556870196045No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT790.1705674065117886No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT680.14681751446584335No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT610.1317039468002418No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT580.12522670351498402No Hit
TGCCATCTTGCCACATCTGTGCCCTGGGCAGGCTCCAAAGGAAAGCTCTG580.12522670351498402No Hit
TGCCATCTTCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT570.1230676224198981No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT540.11659037913464029No Hit
TGCCATCTTGCCAATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT470.10147681146903877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)