FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30557
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG1376045.03059855352293No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC871328.513924796282357No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC312110.213698988775075No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2340.7657819812154335No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1650.5399744739339595No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1620.5301567562260693No Hit
CGAAGTAGACCAGCGGCATCTCGGCCACACCCTGGACCTTAGCTCAGCAC1530.5007036031023988No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1340.438524724285761No Hit
GTTGGCGGATACCCTTCCATAGCAGGGACTGTCTTTCCCTGTTAAGGTTG1240.4057989985927938No Hit
GTTGGCGGATACCCTTCCAAAGCAGGGACTGTCTTTCCCTGTTAAGGTTG1200.3927087083156069No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1110.3632555551919364No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA950.3108943940831888No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG780.2552606604051445No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.22253493471217725No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.19308178158850672No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC580.18980920901921No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT530.17344634617272636No Hit
GTTGGCGGATACCCTTCCATAAAGGCCTTAACACAGAATCAAAAGATTCG410.1341754753411657No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC370.12108518506397879No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACGGAATCAAAAGATTCG360.11781261249468207No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.11126746735608863No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACGCAGAATCAAAAGATTCG320.10472232221749517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)