FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23612
Total Bases3.4 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC756632.04302896832119No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC536422.717262408944606No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG421517.85109266474674No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5752.435202439437574No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3031.2832458072166695No Hit
GTTGGCGGATACCCTTCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGC2010.8512620701338302No Hit
GTTGGCGGATACCCTTCCGGGGCAGGCACAAGATGTGGCAAGATGGCATT1680.7115026257834999No Hit
TACCTCAGTTTGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAG1260.5336269693376249No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT980.4150431983737083No Hit
TACCTCAGTTTGCCCCCTTTCCCCTTTTTTGGAGTAAGATGTGGCAAGAT860.36422158224631546No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA840.35575131289174994No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA790.33457563950533625No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA750.3176351007962053No Hit
TACCTCAGTTTGCCCCCCCTTTTGGCGTCCTAGTAAGATGTGGCAAGATG700.29645942740979164No Hit
TACCTCAGTTTGCCCCCCTAGTAAGATGTGGCAAGATGGCATTGCCATGT650.27528375402337796No Hit
TACCTCAGTTTGCCCCCTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGGC630.26681348466881244No Hit
GTTGGCGGATACCCTTCCAGGGCAGGCACAAGATGTGGCAAGATGGCATT610.258343215314247No Hit
TACCTCAGTTTGCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGGC600.2541080806369643No Hit
GTTGGCGGATACCCTTCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCC570.24140267660511605No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT540.22869727257326783No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA530.2244621378959851No Hit
GTTGGCGGATACCCTTCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCG510.2159918685414196No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA510.2159918685414196No Hit
TACCTCAGTTTGCCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGG500.21175673386413688No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT500.21175673386413688No Hit
GTTGGCGGATACCCTCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCG500.21175673386413688No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG480.20328646450957139No Hit
GTTGGCGGATACCCTTCCTGGAGGAGGTAAGATGTGGCAAGATGGCATTG470.19905132983228865No Hit
GTTGGCGGATACCCTTCCTCGCGCCAGGTAGATGTGGCAAGATGGCATTG440.18634592580044046No Hit
GTTGGCGGATACCCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCG440.18634592580044046No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC400.1694053870913095No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT390.16517025241402677No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.15669998305946128No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT350.14822971370489582No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT300.12705404031848214No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT290.12281890564119939No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC290.12281890564119939No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG290.12281890564119939No Hit
TACCTCAGTTTGCCCCTTTCGTCCTAGTAAGATGTGGCAAGATGGCATTG290.12281890564119939No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC280.11858377096391666No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT280.11858377096391666No Hit
GTTGGCGGATACCCTTCCGGGGCAGGCATAAGATGTGGCAAGATGGCATT270.11434863628663391No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA270.11434863628663391No Hit
TACCTCAGTTTGCCCCCTTTTTTGGTAAGATGTGGCAAGATGGCATTGCC260.11011350160935118No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT260.11011350160935118No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC260.11011350160935118No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA250.10587836693206844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)