FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28735
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG1288144.826866191056205No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC715924.913868105098313No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC328011.414651122324692No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2180.7586566904471899No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1980.6890551592135027No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1710.5950930920480251No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1530.5324517139377066No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1440.5011310248825473No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1200.41760918740212283No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1190.41412911084043846No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA790.2749260483730642No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC720.2505655124412737No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.24708543587958934No Hit
TACCTCAGTTTGCCCCCTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGGC610.21228467026274578No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT570.19836436401600835No Hit
TACCTCAGTTTGCCCCCCTTTTTTGACGTCCTAGTAAGATGTGGCAAGAT520.18096398120758656No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA510.1774839046459022No Hit
TACCTCAGTTTGCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGGC470.1635635983991648No Hit
TACCTCAGTTTGCCCCCTTTTCCCTTTTTTGGCGTCCTAGTAAGATGTGG430.14964329215242736No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT350.12180267965895249No Hit
TACCTCAGTTTGCCCCCTTTTTTGGCGTTCCTAGTAAGATGTGGCAAGAT350.12180267965895249No Hit
TACCTCAGTTTGCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGATG350.12180267965895249No Hit
TACCTCAGTTTGCCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGA320.11136244997389942No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG300.10440229685053071No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACGGAATCAAAAGATTCG300.10440229685053071No Hit
GTTGGCGGATACCCTTCCATAAAGGCCTTAACACAGAATCAAAAGATTCG300.10440229685053071No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAGTCAAAAGATTCG300.10440229685053071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)