FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15730
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA519333.01335028607756No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT429927.32994278448824No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA428127.215511760966304No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT910.5785123966942148No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC800.5085823267641449No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT580.3687221869040051No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT500.3178639542275906No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT490.31150667514303876No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG310.19707565162110616No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT290.18436109345200255No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT290.18436109345200255No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.17800381436745072No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC260.1652892561983471No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.1589319771137953No Hit
TTCTGCAGTTTGGCTAATAGTTGATATATTGAGAGAATCTGGATGATATT230.14621741894469167No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT230.14621741894469167No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT200.12714558169103624No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.12078830260648442No Hit
AAGGTGGAGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT180.11443102352193261No Hit
TTCTGCATTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT160.101716465352829No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT160.101716465352829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)