FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43683
Total Bases6.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1666638.15214156536868No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT841619.266076047890486No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA717016.413707849735594No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC37868.666987157475447No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA29776.815008126731223No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1070.24494654671153537No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT770.1762699448297965No Hit
TGCCATCTTGCCACATCTCTGCCCATTAATTGGAGACTTTACAACATTTA760.17398072476707188No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT600.1373532037634778No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG530.12132866332440538No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAAGGGGGGCAAACTG510.11675022319895612No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT450.10301490282260833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)