FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48318
Total Bases7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1954440.4486940684631No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA831917.217186141810505No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT699714.481145742787366No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC46079.534748954840847No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA31686.556562771637899No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2970.614677759841053No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1880.38908895235729957No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT870.1800571215696014No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGAAAGGGGGCA650.1345254356554493No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT630.1303861914814355No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT590.12210770313340784No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGGGCAAACTGA590.12210770313340784No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGAAAGGGGCAA580.12003808104640093No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGGCAAACTGAG560.1158988368723871No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC560.1158988368723871No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT510.10555072643735255No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT510.10555072643735255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)