FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19787
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT821741.52726537625714No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA592229.928741092636578No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC17438.808813867690908No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA14387.26739778642543No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1570.7934502451104262No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1210.611512609288927No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT750.37903674129478954No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT390.19709910547329054No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT360.18193763582149897No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT350.1768838126042351No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT340.17182998938697125No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT340.17182998938697125No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT310.15666851973517965No Hit
TTCTGCAGTTTGGCTAATAGTTGATATATTGAGAGAATCTGGATGATATT300.15161469651791581No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT300.15161469651791581No Hit
TTCTGCAGTTTGGCTGATAGTTGATATATTGAGAGAATCTGGATGATATT290.14656087330065193No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.1415070500833881No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT270.1364532268661242No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC260.13139940364886035No Hit
TTCTGCATTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT250.12634558043159652No Hit
TTCTGCAGTTTGGCTGAATAGTTGCTATATTGAGAGAATCTGGATGATAT250.12634558043159652No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT240.12129175721433264No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC220.11118411077980493No Hit
TTCTGCAGTTTGGCGAATAGTTGATATATTGAGAGAATCTGGATGATATT220.11118411077980493No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGGTGATAT210.10613028756254106No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT200.10107646434527721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)