FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47783
Total Bases6.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2010342.071448004520434No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA825617.278111462235522No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT515710.79254128037168No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC483910.127032626666388No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA34947.31222401272419No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG6571.3749659920892368No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4110.8601385429964631No Hit
TGCCATCTTGCCACATCTGTGTCAGAGGCATCCTTACCTAACTCACAATT1250.2615993135634012No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC1140.2385785739698219No Hit
TGCCATCTTGCCACATCTTATAACGGGGTGGGGGTGGGAATCATCACAAA950.1988154783081849No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT750.15695958813804073No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT740.1548667936295335No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT580.12138208149341816No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC550.11510369796789653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)