FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15642
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA623339.84784554404808No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA378524.197672931850146No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT339021.672420406597624No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA2081.3297532284874058No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT760.48587137194732133No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT590.37718961769594683No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT530.3388313514895794No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC480.3068661296509398No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT390.24932873034138858No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT380.24293568597366066No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG330.21097046413502107No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.1981843753995653No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT290.18539828666410946No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.17900524229638154No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC260.16621915356092573No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC240.1534330648254699No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA230.14704002045774198No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT210.13425393172228614No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG210.13425393172228614No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT190.12146784298683033No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT180.11507479861910241No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT170.1086817542513745No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT170.1086817542513745No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA160.1022887098836466No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA160.1022887098836466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)