FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12366
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG572146.26394953905871No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC345627.947598253275107No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC10378.385896813844413No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA2341.8922852983988356No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA950.7682354843926896No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA950.7682354843926896No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA830.6711952126799289No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.38816108685104317No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.38816108685104317No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA470.38007439754164646No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA460.37198770823224975No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA300.24260067928190196No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC220.1779071648067281No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA220.1779071648067281No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACGGAATCAAAAGATTCG200.16173378618793466No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACGCAGAATCAAAAGATTCG190.15364709687853792No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC170.13747371825974444No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT160.12938702895034773No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT160.12938702895034773No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT150.12130033964095098No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGGTTCG150.12130033964095098No Hit
GTTGGCGGATACCCTCCCATAAAGGCTTTAACACAGAATCAAAAGATTCG140.11321365033155426No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAGAGATTCG130.10512696102215754No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAGTCAAAAGATTCG130.10512696102215754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)