FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48230
Total Bases6.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2260146.86087497408252No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA805116.692929711797635No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT646113.39622641509434No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC572211.863985071532241No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2660.5515239477503628No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1560.3234501347708895No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT760.15757827078581796No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT610.12647729628861704No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGAAAGGGGCAA560.11611030478955006No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT550.11403690648973668No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT520.1078167115902965No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT510.1057433132904831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)