FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35771
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT929825.993122920801763No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA702819.647200246009337No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT684119.124430404517625No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA375510.497330239579547No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC28748.03444130720416No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA7342.0519415168712083No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC6211.7360431634564313No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1830.5115875988929579No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACCAGTC1260.3522406418607252No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1080.30192055016633584No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACAGTCAA980.2739649436694529No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC950.265578261720388No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACCAGTCAA860.24041821587319337No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACCAGTCAA800.2236448519750636No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT650.18171144222973917No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT570.1593469570322328No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACCAGTCAA560.1565513963825445No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCACCAGTCAA470.13139135053534987No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT430.12020910793659668No Hit
TGCCATCTTGCCACATCTGCCAATAGCTCCCAGTCCCCTGGCTACAAATC370.1034357440384669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)