FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44880
Total Bases6.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1926042.9144385026738No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT582012.967914438502673No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA473510.55035650623886No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC43169.61675579322638No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCAGTC31356.985294117647059No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA15483.449197860962567No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC2800.6238859180035651No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1800.4010695187165776No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCACAGT1290.2874331550802139No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGGCAAACTGAG1120.24955436720142604No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1080.24064171122994654No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1060.23618538324420676No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACCAGTC1040.23172905525846704No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGGGGCAAACTG990.22058823529411764No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT660.14705882352941177No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT640.14260249554367202No Hit
TGCCATCTTGCCACATCTTACTAGGACGAAAAAAGGGGGCAAACTGAGGT610.1359180035650624No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT530.11809269162210338No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT510.11363636363636363No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT500.11140819964349377No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT490.10918003565062388No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT490.10918003565062388No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC460.10249554367201426No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT450.1002673796791444No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)