FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50808
Total Bases7.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA2301545.29798456935916No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG949518.68800188946623No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC616812.139820500708549No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC36037.091402928672649No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG2390.4703983624626043No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2250.44284364666981574No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1940.38182963312864115No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1770.3483703353802551No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1720.33852936545425916No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1650.32475200755786493No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1560.30703826169107223No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1540.30310187372067393No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1470.28932451582427965No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1260.24799244213509686No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1170.23027869626830422No Hit
TACCTCAGTTTGCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGGC1030.20272398047551568No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG990.19485120453471894No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG970.19091481656432058No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA930.18304204062352386No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG860.16926468272712958No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA780.15351913084553612No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT730.14367816091954022No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.13974177294914186No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA640.1259644150527476No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA630.12399622106754843No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA590.1161234451267517No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA580.1141552511415525No Hit
TACCTCAGTTTGCCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGA570.11218705715635334No Hit
GTTGGCGGATACCCTTCCATAAAGGCGTTAACACAGAATCAAAAGATTCG530.10431428121555661No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA530.10431428121555661No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA520.10234608723035743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)