FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13979
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA454132.48444094713499No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA339424.279276056942557No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT12929.24243508119322No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA5764.120466413906574No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC4353.111810573002361No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT4022.8757421847056297No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA1731.2375706416768009Clontech Universal Primer Mix Long (96% over 25bp)
AAGGTGGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGA1190.8512769153730596No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1120.8012018027040559No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC1010.7225123399384792No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1000.7153587524143358No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG820.5865941769797554No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT750.5365190643107518No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT620.44352242649688817No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT430.3076042635381644No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT380.2718363259174476No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA370.26468273839330425No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC330.2360683882967308No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA320.22891480077258747No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC240.1716861005794406No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT220.15737892553115387No Hit
TGCCATCTTGCCACATCTTGTTTCCTTGAACTACACAGAAAGCAGTGGTA220.15737892553115387No Hit
TGCCATCTTGCCACATCTTAGTCATCTGTCATTCTTCCAGGAGTTAAAGC210.15022533800701052No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.1359181629587238No Hit
TGCCATCTTGCCACATCTTCCTCCCTAAGCAGTGGTATCAACGCAGAGTA180.12876457543458045Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGGGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT180.12876457543458045No Hit
TGCCATCTTGCCACATCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC180.12876457543458045No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACCAGTCAA160.11445740038629373No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC150.10730381286215038No Hit
AAGGTGGATGGTGATGTGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT150.10730381286215038No Hit
TGCCATCTTGCCACATCTTTCCTTAAGCAGTGGTATCAACGCAGAGTACA140.10015022533800699Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCCGCTTCCGGGCTGA140.10015022533800699No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)