FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20824
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA699733.60065309258548No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT459122.046676911256245No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA383818.43065693430657No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT17028.1732616212063No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12255.88263542066846No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT890.4273914713791778No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG700.3361505954667691No Hit
TGCCATCTTGCCACATCTTAGGGAGCAACTGTGTGAGATGAGTTCAGGTG650.31213983864771416No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT550.2641183250096043No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG500.24010756819054938No Hit
TGCCATCTTGCCACATCTTCTGATCTGTCCTTACCTTCTCCGTGTTTTCC470.22570111409911642No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG420.20169035728006146No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT410.19688820591625047No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.17287744909719555No Hit
TGCCATCTTGCCACATCTTTCTATGTATTTATTTCAACTCTCCTTACTCA300.14406454091432963No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT300.14406454091432963No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.13446023818670766No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC260.12485593545908567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)