FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42398
Total Bases6.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1806542.60814189348554No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC550112.974668616444172No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA497411.731685456861172No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA429210.12311901504788No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT34598.158403698287655No Hit
TGCCATCTTGCCACATCTTGCTTGTGGCCCATTTCAAGTTCTATAATTTG3770.8891928864569083No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3620.8538138591442992No Hit
TGCCATCTTGCCACATCTTTCAGTGAGTAGTAGGAAGAATGTAGGATACC2800.6604085098353696No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2030.478796169630643No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1130.2665220057549884No Hit
TGCCATCTTGCCACATCTATTGCTTCGAATTGTGGGAGGAGGGAAGGGTA990.23350158026321996No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT750.17689513656304542No Hit
TGCCATCTTGCCACATCTTGCGGCACATGGACCTGAACCATGCGCGTCTA750.17689513656304542No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT720.1698193311005236No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT630.14859191471295816No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC540.1273644983253927No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT540.1273644983253927No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT500.11793009104203028No Hit
TGCCATCTTGCCACATCTTACTTGTGGCCCATTTCAAGTTCTATAATTTG500.11793009104203028No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT490.11557148922118968No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT460.10849568375866786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)