FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35776
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1473541.18682915921288No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA614517.17631932021467No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC512214.31686046511628No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA28798.04729427549195No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT24476.839780858676208No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1830.5115161001788908No Hit
TGCCATCTTGCCACATCTTGTCTCAAACTGTTGGGCTGAAGCAGTTCTCC1290.3605769230769231No Hit
TGCCATCTTGCCACATCTAGTCTCAAACTGTTGGGCTGAAGCAGTTCTCC1240.3466010733452594No Hit
TGCCATCTTGCCACATCTGGAATGGAAGGGTATCCGCCAACCAGTCAAGA910.2543604651162791No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG860.2403846153846154No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT790.22081842576028624No Hit
TGCCATCTTGCCACATCTTGAATGGAAGGGTATCCGCCAACCAGTCAAGA710.19845706618962433No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT640.17889087656529518No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT480.13416815742397137No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT480.13416815742397137No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT460.1285778175313059No Hit
TGCCATCTTGCCACATCTCTACGCATTTCACCGAAGGGTATCCGCCAACC390.10901162790697674No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT370.10342128801431127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)