FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35776
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1071429.947450805008945No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG642817.967352415026834No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC527614.74731663685152No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC23466.557468694096601No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA4821.3472719141323792No Hit
GTTGGCGGATACCCTTCCACACACGCAAATACTCCTTCAGCGGAGCGAAG4801.3416815742397137No Hit
GTTGGCGGATACCCTTCCTCTCTCGGTGTCTCTGTTCTGTCTCTGCTAAG2110.5897808586762074No Hit
GTTGGCGGATACCCTTCCATACACGCAAATACTCCTTCAGCGGAGCGAAG1730.48356440071556345No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA1570.43884168157423975No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1500.41927549194991054No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1360.38014311270125223No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1320.3689624329159213No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1260.35219141323792486No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1000.27951699463327373No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA970.2711314847942755No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC850.23758944543828264No Hit
GTTGGCGGATACCCTTCCATTCCAGATGTGGCAAGATGGCATTGCCATGT800.22361359570661896No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG730.2040474060822898No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG720.20125223613595708No Hit
GTTGGCGGATACCCTTACACACACGCAAAATACTCCTTCAGCGGAGCGAA710.19845706618962433No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA640.17889087656529518No Hit
GTTGGCGGATACCCTTCCATTCAAGATGTGGCAAGATGGCATTGCCATGT610.17050536672629696No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA580.16211985688729874No Hit
GTTGGCGGATACCCTTACACACACGCAAATACTCCTTCAGCGGAGCGAAG540.1509391771019678No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG520.14534883720930233No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG510.14255366726296959No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA510.14255366726296959No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACCCGCAAAATACTCCTTCA480.13416815742397137No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATACTCCTTCA480.13416815742397137No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC470.13137298747763865No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.13137298747763865No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.13137298747763865No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA440.12298747763864043No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT370.10342128801431127No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG370.10342128801431127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)