FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008154883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66176375
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG2515550.3801281046294845TruSeq Adapter, Index 7 (97% over 35bp)
GCAGATCTTTTCGCACCAGCTCTTTGAATTCCCCCTGGTTCAGGGTGTCTG1154020.17438549633460582No Hit
GCACCATCAGTGTTGATATCCAACTCTTTGAACCAGACGTCTGCACCCTTT896740.1355075735109395No Hit
CTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCGTG850090.1284582299952211No Hit
CCCTCGTGCATCTTCTCGTGGGAGGCCCAGGTTAGCCTCGCCATCAGCATG790600.11946861701022458No Hit
GTCCAGGTCCTCCATGATGTGTTCTATGACCTTTTCATTCTTATTCTCCTT724240.10944086919236662No Hit
CTCGTGCATCTTCTCGTGGGAGGCCCAGGTTAGCCTCGCCATCAGCATGAT705820.10665739850513117No Hit
CTCCTGATTAGTGGCTGTGGCCGTGGCCACCATGACTGTGGCCGTGGCCGT681970.10305339329934587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)