FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008154984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62446414
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG4235120.678200672980197TruSeq Adapter, Index 13 (97% over 38bp)
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA1245320.19942217979082036No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTATG1185380.18982355015613867TruSeq Adapter, Index 13 (97% over 38bp)
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAG1115620.17865237225631564No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCAC891240.14272076535892037No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGC882720.14135639558101767No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAG848100.13581244232855388No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG831500.13315416318381387No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTT793050.1269968840804854No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACAC724250.11597943798662322No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA723600.11587534874300388No Hit
CGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG675300.10814071725559772No Hit
GTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCT672810.10774197538388675No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT665410.10655695937960505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)