FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008155151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58190665
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG23539084.045164288808179TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTATG5734570.985479371992054TruSeq Adapter, Index 27 (97% over 39bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC1179470.2026905861962567No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC881300.15145040875542495No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGA837120.14385812569765272No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA663960.11410077544224662No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAG637690.10958630563854185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)