FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008155209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79315237
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG14263011.7982685974953339TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTATG2198230.27715103467445984TruSeq Adapter, Index 13 (97% over 38bp)
CTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCT1038510.13093448866577803No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG951540.1199693824277421No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACAC825920.10413131590339951No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC800010.1008646043634718No Hit
CCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCTTG793690.10006778394925556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)