FastQCFastQC Report
Thu 9 May 2024
EGAF00008155417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70278097
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT8349021.1879974496179087TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT1887960.2686413093968666TruSeq Adapter, Index 7 (97% over 36bp)
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC949340.13508333898113375No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG763930.10870100822451126No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA758350.10790701973617754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)