FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008155420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67885918
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG2993190.4409147122382583TruSeq Adapter, Index 2 (97% over 37bp)
GCACCATCAGTGTTGATATCCAACTCTTTGAACCAGACGTCTGCACCCTTT1771970.2610217335501009No Hit
CCCTCGTGCATCTTCTCGTGGGAGGCCCAGGTTAGCCTCGCCATCAGCATG1507820.2221108654669736No Hit
CCCTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCG1482280.2183486713695173No Hit
GCAGATCTTTTCGCACCAGCTCTTTGAATTCCCCCTGGTTCAGGGTGTCTG1474380.2171849543229275No Hit
GTCCAGGTCCTCCATGATGTGTTCTATGACCTTTTCATTCTTATTCTCCTT1036500.1526826226316922No Hit
CTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCGTG938210.13820392028874087No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTATG907920.13374202290377807TruSeq Adapter, Index 2 (97% over 37bp)
CCTCGTGCATCTTCTCGTGGGAGGCCCAGGTTAGCCTCGCCATCAGCATGA777610.11454658387325631No Hit
CTCCATGATGTGTTCTATGACCTTTTCATTCTTATTCTCCTTCTTGAGAAA769350.11332983668277123No Hit
GCATCTTCTCGTGGGAGGCCCAGGTTAGCCTCGCCATCAGCATGATGAACT710740.10469623464471674No Hit
CCTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCGT700500.10318782166280789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)