FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008155442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64402052
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG2106420.32707342927520383TruSeq Adapter, Index 3 (97% over 36bp)
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA1519480.2359365816480506No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG1166100.18106565921222512No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCAC1076270.1671173458882956No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGC945880.14687109659176698No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT897280.1393247531926467No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACAC835210.12968686153043696No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTATG773790.12014989832932653TruSeq Adapter, Index 3 (97% over 36bp)
CGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG715460.11109273350482683No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTT690650.10724037178194261No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC648600.10071107672159266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)