FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008155518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68209636
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG1976490.28976697661896333TruSeq Adapter, Index 7 (97% over 35bp)
GCACCATCAGTGTTGATATCCAACTCTTTGAACCAGACGTCTGCACCCTTT1514350.2220140861036115No Hit
GCAGATCTTTTCGCACCAGCTCTTTGAATTCCCCCTGGTTCAGGGTGTCTG1223920.1794350581199407No Hit
GTCCAGGTCCTCCATGATGTGTTCTATGACCTTTTCATTCTTATTCTCCTT1135240.16643396249761544No Hit
CTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCGTG979640.14362193634928647No Hit
CCCTCGTGCATCTTCTCGTGGGAGGCCCAGGTTAGCCTCGCCATCAGCATG863910.1266551253843372No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTATG810580.11883658197501597TruSeq Adapter, Index 7 (97% over 35bp)
CTTAGGGGGTGCCCTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCT736590.10798914100641148No Hit
CCCTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCG689220.10104437443413421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)