FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008155536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66360344
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG5509580.8302518745231339TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTATG1448780.21832014614029124TruSeq Adapter, Index 7 (97% over 35bp)
GCAGATCTTTTCGCACCAGCTCTTTGAATTCCCCCTGGTTCAGGGTGTCTG924840.1393663661538584No Hit
CTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCGTG844000.12718439193142217No Hit
CCCTCGTGCATCTTCTCGTGGGAGGCCCAGGTTAGCCTCGCCATCAGCATG701530.1057152446346571No Hit
CCCTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCG698760.10529782666587745No Hit
GTCCAGGTCCTCCATGATGTGTTCTATGACCTTTTCATTCTTATTCTCCTT671560.1011989931818316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)