FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008155694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62986983
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG2793750.44354402559652684TruSeq Adapter, Index 27 (97% over 39bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC1001310.15897094166266068No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGA837010.1328861869761249No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC836810.13285443438368846No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTATG801090.1271834213745402TruSeq Adapter, Index 27 (97% over 39bp)
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA783030.12431616227752962No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAA758320.12039312948200742No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAG665810.10570596785053192No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTG661200.10497407059487196No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCG659350.10468035911483488No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG657900.10445015281967068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)