FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008155879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008155879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65235
Total Bases7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGTTCTTCTGCTGTGCCCTTGATGC2100.3219130834674638No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG2080.31884724457729746No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC2000.3065838890166322No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG1750.2682609028895532No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1700.2605963056641374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG1410.2161416417567257No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1340.20541120564114357No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1030.15789070284356557Clontech Universal Primer Mix Long (96% over 26bp)
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1020.15635778339848241No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA1000.1532919445083161No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATC850.1302981528320687No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATC820.1256993944968192No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG810.12416647505173603Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG770.11803479727140338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT720.11037020004598759Clontech Universal Primer Mix Long (96% over 26bp)
CGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG680.10423852226565496No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA660.10117268337548863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)