FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559036
Total Bases448.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG40550.15845810688087233No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA37330.14587524364643562No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC34960.13661394368817006No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC34650.13540255002274293No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA30670.11984981844725905No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA28260.1104322096289384No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT26830.10484416788196806No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG26410.10320292485138935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)