FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38082
Total Bases6.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4701.234178877159813No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4461.1711569770495247No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2180.5724489260017857No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1700.44640512578120894No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1420.37287957565253926No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1390.36500183813875325No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1320.34662045060658575No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1280.33611680058820437No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1260.3308649755790137No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1250.32823906307441836No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1220.3203613255606323No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1220.3203613255606323No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1150.3019799380284649No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1120.29410220051467884No Hit
TATCAACGCAGAGTACATGGGGGGG1100.28885037550548814No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1060.27834672548710676No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1050.27572081298251144No Hit
GTATCAACGCAGAGTACATGGGGAG1040.27309490047791607No Hit
GGTATCAACGCAGAGTACATGGGGG1020.2678430754687254No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA960.2520876004411533No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA910.23895803791817655No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT900.2363321254135812No Hit
GTATCAACGCAGAGTACATGGGGGG890.23370621290898586No Hit
GTATCAACGCAGAGTACATGGGGGGG820.21532482537681844No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG820.21532482537681844No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA810.2126989128722231No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT790.2074470878630324No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA780.20482117535843705No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG770.2021952628538417No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG690.18118796281707894No Hit
GTATCAACGCAGAGTACATGGGGTG680.1785620503124836No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT640.16805840029410218No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT630.16543248778950684No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG620.1628065752849115No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA610.16018066278031615No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA590.15492883777112545No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC570.14967701276193476No Hit
GTATCAACGCAGAGTACATGGAGAG570.14967701276193476No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT570.14967701276193476No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA570.14967701276193476No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG550.14442518775274407No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG550.14442518775274407No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC550.14442518775274407No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT540.14179927524814873No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT530.13917336274355338No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT530.13917336274355338No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG520.13654745023895803No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT520.13654745023895803No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC500.13129562522976734No Hit
ATTTAAATCCCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCA500.13129562522976734No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT490.128669712725172No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA490.128669712725172No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG490.128669712725172No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA480.12604380022057665No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA470.12341788771598129No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG470.12341788771598129No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA470.12341788771598129No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT470.12341788771598129No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG460.12079197521138595No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG460.12079197521138595No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT440.11554015020219525No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC430.11291423769759991No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT430.11291423769759991No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG430.11291423769759991No Hit
CTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAA420.11028832519300456No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC420.11028832519300456No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC420.11028832519300456No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT410.10766241268840922No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT410.10766241268840922No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG410.10766241268840922No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA400.10503650018381387No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC400.10503650018381387No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC390.10241058767921853No Hit
GTATCAACGCAGAGTACATGGGGATGGGG390.10241058767921853No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT390.10241058767921853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)