FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21102
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGGGGG1310.620794237513032No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1150.5449720405648754No Hit
GTATCAACGCAGAGTACATGGGGGGG920.4359776324519003No Hit
GTATCAACGCAGAGTACATGGGGGG850.4028054212870818No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC850.4028054212870818No Hit
GGTATCAACGCAGAGTACATGGGGG750.35541654819448393No Hit
GTATCAACGCAGAGTACATGGGGTG530.2511610273907687No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC450.21324992891669034No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA430.2037721542981708No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA430.2037721542981708No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT420.199033266988911No Hit
GTATCAACGCAGAGTACATGGGGAG410.1942943796796512No Hit
GGTATCAACGCAGAGTACATGGGGGGG400.18955549237039143No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA390.18481660506113165No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG340.16112216851483271No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT290.13742773196853378No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA280.132688844659274No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC270.12794995735001422No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA250.11847218273149465No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC250.11847218273149465No Hit
GTATCAACGCAGAGTACATGGGATG250.11847218273149465No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA250.11847218273149465No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT240.11373329542223486No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT240.11373329542223486No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT240.11373329542223486No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG230.10899440811297507No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA220.1042555208037153No Hit
GGTATCAACGCAGAGTACATGGGGGG220.1042555208037153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)