FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008156301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008156301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18356
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2841.5471780344301589No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2591.410982784920462No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1450.7899324471562432No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1280.6973196774896492No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG940.5120941381564611No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA910.49575070821529743No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG870.4739594682937459No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC870.4739594682937459No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA860.468511658313358No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA840.45761603835258224No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT830.45216822837219434No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG790.4303769884506428No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT750.40858574852909135No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT750.40858574852909135No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA740.40313793854870345No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT720.3922423185879277No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG650.3541076487252125No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT630.3432120287644367No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT620.33776421878404883No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT590.32142078884288516No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC560.3050773589017215No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT540.29418173894094574No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG530.2887339289605579No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG530.2887339289605579No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT520.28328611898017No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA500.2723904990193942No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT460.25059925909784264No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT450.2451514491174548No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT450.2451514491174548No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG440.23970363913706688No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC430.234255829156679No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT430.234255829156679No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC420.22880801917629112No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG420.22880801917629112No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT390.2124645892351275No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG390.2124645892351275No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA390.2124645892351275No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC390.2124645892351275No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC390.2124645892351275No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT380.2070167792547396No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA380.2070167792547396No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC380.2070167792547396No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG360.19612115929396384No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT360.19612115929396384No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG340.18522553933318806No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA340.18522553933318806No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC340.18522553933318806No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG330.17977772935280018No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT330.17977772935280018No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT330.17977772935280018No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG320.1743299193724123No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG320.1743299193724123No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT310.16888210939202442No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG310.16888210939202442No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG300.1634342994116365No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC300.1634342994116365No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA300.1634342994116365No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC290.15798648943124863No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG280.15253867945086075No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA280.15253867945086075No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC280.15253867945086075No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT270.14709086947047287No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA270.14709086947047287No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA270.14709086947047287No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG270.14709086947047287No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC270.14709086947047287No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG260.141643059490085No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT260.141643059490085No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC260.141643059490085No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT250.1361952495096971No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC250.1361952495096971No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA250.1361952495096971No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC250.1361952495096971No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA240.13074743952930923No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA240.13074743952930923No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG240.13074743952930923No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG240.13074743952930923No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT240.13074743952930923No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC240.13074743952930923No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG240.13074743952930923No Hit
TCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGG230.12529962954892132No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT230.12529962954892132No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA230.12529962954892132No Hit
AGCACGATCGGCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGG230.12529962954892132No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC230.12529962954892132No Hit
GTTATAAGACTGACTAGCCCGATGGGCCTCCCAACGGGCCCTCCTCCCCT230.12529962954892132No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG230.12529962954892132No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT230.12529962954892132No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG230.12529962954892132No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC230.12529962954892132No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG230.12529962954892132No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG220.11985181956853344No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG220.11985181956853344No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA220.11985181956853344No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG220.11985181956853344No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC210.11440400958814556No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT210.11440400958814556No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA210.11440400958814556No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC210.11440400958814556No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT210.11440400958814556No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT210.11440400958814556No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT210.11440400958814556No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC210.11440400958814556No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT200.10895619960775767No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT200.10895619960775767No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC200.10895619960775767No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT200.10895619960775767No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG200.10895619960775767No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC200.10895619960775767No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA200.10895619960775767No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC200.10895619960775767No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT190.1035083896273698No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC190.1035083896273698No Hit
ATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGG190.1035083896273698No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC190.1035083896273698No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA190.1035083896273698No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA190.1035083896273698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)